具有Cap 1结构的萤火虫荧光素酶mRNA,提供更高的转录效率。
<strong>Background</strong>
EZ Cap™ Firefly Luciferase mRNA will express luciferase protein once entering cells, which is initially extracted from firefly Photinus pyralis. This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc.
EZ Cap™ Firefly Luciferase mRNA is provided at a concentration of 1mg/ml. It is co-transcriptional capped by EZ Cap™ Reagent AG (3' OMe) (Catalog No.B8178) which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). EZ Cap™ Reagent AG (3' OMe) obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with EZ Cap™ Reagent AG. The addition of poly (A) tail increases the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
 
All the modifications are intention to mimics a fully processed mature mRNA. EZ Cap™ Firefly Luciferase mRNA is an ideal product to observing mRNA delivery, translation and other behaviors.
文献引用
1. Jiaxing Di, Zhili Du, et al. "Biodistribution and Non-linear Gene Expression of mRNA LNPs Affected by Delivery Route and Particle Size." Pharm Res. 2022 Jan;39(1):105-114. PMID:35080707
2. Min Li , Sanpeng Li, et al. "Secreted Expression of mRNA-Encoded Truncated ACE2 Variants for SARS-CoV-2 via Lipid-Like Nanoassemblies." Adv Mater. 2021 Aug;33(34):e2101707. PMID:34278613
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<h2 style="box-sizing: border-box; outline: none; line-height: 1.3; font-size: 1.3em; margin: 0px; padding: 0px; color: #363f4d;">Description</h2>
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<td class="font-bold" style="box-sizing: border-box; outline: none; padding: 8px 10px; margin: 0px; vertical-align: top; border-bottom: 1px solid #dddddd; border-right: 1px solid #dddddd; word-break: break-word;">General tips</td>
<td style="box-sizing: border-box; outline: none; padding: 8px 10px; margin: 0px; vertical-align: top; border-bottom: 1px solid #dddddd; border-right: 1px solid #dddddd; word-break: break-word;" colspan="3">请将其于冰上溶解,并小心防止RNase污染降解。 尽可能避免反复冻融。 不要涡旋震荡。 首次使用时,将其轻柔离心并分成几份,可供单独使用。 使用不含RNase的试剂和耗材,使用适当的无RNase技术。 直至与转染试剂混合,才可加入含有血清的培养基中。</td>
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